LOCUS       UGT1A7-pGEc2            6687 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(6165..6294)
                     /note="3'-ITR"
     misc_feature    complement(5863..6077)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    5315..5856
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    4494..4516
                     /note="attB2"
     misc_feature    5294..5299
                     /note="BstB1 site"
     misc_feature    4517..5212
                     /note="Super GFP"
     misc_feature    5213..5263
                     /note="AviTag"
     misc_feature    5264..5287
                     /note="His Tag"
     misc_feature    3011..4471
                     /note="OPTIMIZED UGT1A7 coding region"
     PCR_frame       3011..4493
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    4472..4492
                     /note="TEV"
     vector          join(4502..6687,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..4501
                     /source="UGT1A7 pDONR"
                     /type="Entry Clone"
     source          1..6687
                     /dnas_title="UGT1A7-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGGCCAGAG CTGGATGGAC AGGACTGCTG CCTCTGTACG TGTGTCTGCT
     3061 GCTGACCTGT GGATTTGCCA AGGCCGGAAA ACTGCTGGTG GTGCCTATGG ATGGCAGCCA
     3121 CTGGTTTACC ATGCAGAGCG TGGTGGAAAA GCTGATCCTG AGAGGACACG AGGTGGTGGT
     3181 CGTGATGCCT GAAGTGTCTT GGCAGCTGGG CAGAAGCCTG AACTGCACCG TCAAGACCTA
     3241 CAGCACCAGC TACACCCTGG AAGATCAGGA CCGCGAGTTC ATGGTGTTCG CCGATGCTAG
     3301 ATGGACCGCC CCTCTGAGAT CTGCCTTTAG CCTGCTGACA AGCAGCAGCA ACGGCATCTT
     3361 CGATCTGTTC TTCAGCAACT GCCGGTCCCT GTTCAACGAC CGGAAGCTGG TGGAATACCT
     3421 GAAAGAGTCC TGCTTCGACG CCGTGTTCCT GGACCCTTTT GACGCCTGTG GACTGATCGT
     3481 GGCCAAGTAC TTCAGCCTGC CCTCCGTGGT GTTTGCCAGA GGCATCTTTT GCCACTATCT
     3541 GGAAGAGGGC GCCCAGTGTC CTGCCCCACT GTCTTATGTG CCTAGACTGC TCCTGGGCTT
     3601 CAGCGACGCC ATGACCTTCA AAGAAAGAGT GTGGAACCAC ATCATGCACC TCGAGGAACA
     3661 CCTGTTCTGC CCCTACTTCT TCAAGAACGT GCTGGAAATC GCCAGCGAGA TCCTGCAGAC
     3721 ACCTGTGACC GCCTACGATC TGTACAGCCA CACCAGCATC TGGCTGCTGA GAACCGACTT
     3781 CGTGCTCGAG TACCCTAAGC CTGTGATGCC CAACATGATC TTCATCGGCG GCATCAACTG
     3841 CCACCAGGGC AAGCCCGTGC CAATGGAATT CGAGGCCTAT ATCAATGCCA GCGGCGAGCA
     3901 CGGCATTGTG GTGTTCAGTC TGGGCAGCAT GGTGTCCGAG ATCCCCGAGA AAAAGGCCAT
     3961 GGCTATTGCC GACGCCCTGG GCAAGATTCC TCAGACAGTG CTGTGGCGGT ACACCGGCAC
     4021 AAGACCAAGC AACCTGGCCA ACAACACCAT CCTGGTCAAA TGGCTGCCCC AGAACGATCT
     4081 GCTGGGCCAC CCTATGACCA GAGCCTTTAT CACACACGCC GGCTCTCACG GCGTGTACGA
     4141 GTCTATCTGT AATGGCGTGC CCATGGTGAT GATGCCCCTG TTCGGCGACC AGATGGACAA
     4201 CGCCAAGCGG ATGGAAACAA AGGGCGCTGG CGTGACCCTG AATGTCCTGG AAATGACCTC
     4261 CGAGGACCTG GAAAATGCCC TGAAGGCCGT GATCAACGAC AAGAGCTACA AAGAAAACAT
     4321 CATGAGACTG AGCAGCCTGC ACAAGGACAG ACCCGTGGAA CCTCTGGACC TGGCCGTGTT
     4381 TTGGGTGGAA TTTGTGATGC GGCACAAAGG CGCCCCTCAT CTGAGGCCTG CTGCTCACGA
     4441 TCTGACCTGG TATCAGTACC ACTCCCTGGA Cgaaaacttg tactttcaag gctCCAGCTT
     4501 TCTTGTACAA AGTGGTATGA GCAAGGGCGA GGAACTGTTC ACCGGCGTGG TGCCCATCCT
     4561 GGTGGAACTG GATGGCGACG TGAACGGCCA CAAGTTCTCT GTGCGGGGAG AGGGCGAAGG
     4621 CGACGCCACA AATGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT
     4681 GCCTTGGCCT ACCCTCGTGA CCACACTGAC CTACGGCGTG CAGTGCTTCA GCAGATACCC
     4741 CGACCATATG AAGCGGCACG ACTTCTTCAA GAGCGCCATG CCCGAGGGCT ACGTGCAGGA
     4801 ACGGACCATC AGCTTCAAGG ACGACGGCAC CTACAAGACC AGAGCCGAAG TGAAGTTCGA
     4861 GGGCGACACC CTCGTGAACC GGATCGAGCT GAAGGGCATC GATTTCAAAG AGGACGGCAA
     4921 CATCCTGGGC CACAAGCTGG AGTACAACTT CAACAGCCAC AACGTGTACA TCACCGCCGA
     4981 CAAGCAGAAG AACGGCATCA AGGCCAACTT CAAGATCCGG CACAACGTGG AAGATGGCAG
     5041 CGTGCAGCTG GCCGACCACT ACCAGCAGAA CACCCCCATC GGAGATGGCC CCGTGCTGCT
     5101 GCCCGACAAC CACTACCTGA GCACCCAGAG CGTGCTGAGC AAGGACCCCA ACGAGAAGCG
     5161 GGACCACATG GTGCTGCTGG AATTCGTGAC CGCCGCTGGC ATCACCCACG GCATGAGCGG
     5221 CCTGAACGAC ATCTTCGAGG CCCAGAAAAT CGAGTGGCAC GAGCACCACC ATCATCACCA
     5281 TCACCACTGA TGATTCGAAA AATAATAAGG ATCCAATCAA CCTCTGGATT ACAAAATTTG
     5341 TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC
     5401 TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA
     5461 TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT
     5521 GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA
     5581 GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC
     5641 CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT
     5701 GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG
     5761 CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG
     5821 CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGAGAT CTGCCTCGAC TGTGCCTTCT
     5881 AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC
     5941 ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT
     6001 CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT
     6061 AGCAGGCATG CTGGGGACTC GAGTTAAGGG CGAATTGCCG ATTAGGATCT TCCTAGAGCA
     6121 TGGCTACGTA GATAAGTAGC ATGGCGGGTT AATCATTAAC TACAAGGAAC CCCTAGTGAT
     6181 GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG CTCGCTCACT GAGGCCGGGC GACCAAAGGT
     6241 CGCCCGACGC CCGGGCTTTG CCCGGGCGGC CTCAGTGAGC GAGCGAGCGC GCAGCCTTAA
     6301 TTAAGAGCTC ATGGCGCGCC TAGGCCTTGA CGGCCTTCCG CCAATTCGCC CTATAGTGAG
     6361 TCGTATTACG TCGCGCTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG
     6421 CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA
     6481 AGAGGCCCGC ACCGAAACGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGGAGC
     6541 GCCCTGTAGC GGCCACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG
     6601 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT
     6661 AACAAAATAT TAACGCTTAC AATTTAG
//

